Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 0.5M HEPES, pH 7.5. 1000mL, Sterile. 2 pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Medchemexpress LLC 1-Piperazinepropanesulfonic acid, β-hydroxy-4-(2-hydroxyethyl)- | 68399-78-0 | 99.9% | 50 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It has a pKa of 7.84 at 2.0 mM buffer concentration and exhibits a relatively high ability to bind copper(II). It is for research use only.
- Zwitterionic buffer
- Working pH range: 7.1-8.5
- pKa of 7.84 at 2.0 mM buffer concentration
- Relatively high ability to bind copper(II)
Medchemexpress LLC 3-(N-morpholino)propanesulfonic acid sodium salt | 71119-22-7 | 99.8% | 100 G
MOPS sodium salt acts as a buffering agent within biological contexts. This buffer is critical for sustaining the appropriate pH levels in mammalian cell culture media. It also significantly enhances the thermal stability of bovine serum albumin.
- Functions as a buffering agent.
- Maintains pH of mammalian cell culture media.
- Improves thermal stability of bovine serum albumin.
Sigma Aldrich Fine Chemicals Biosciences HEPES, 7365-45-9, MFCD00006158, 100g
Empirical Formula (Hill Notation) C8H18N2O4S, ≥99.5% (titration), Molecular Weight 238.30, Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Bioworld ACES Buffer, 0.5M, pH 6.5, 100 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 6.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 7.0) - 250ml
HEPES Buffer (0 5 M pH 7 0) - 250ml (For Research Use and Further Manufacturing Only)
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 100 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255310.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 100 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurineACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agentinbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+.Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.0
Medchemexpress LLC PIPES (1,4-Piperazinediethanesulfonic acid) | 5625-37-6 | ≥99.0% | 5 G
PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry. It is for research use only.
- Important component of buffer agent
- Prevents glutaraldehyde fixation-induced lipid loss and artifacts when used in buffer
Hampton Research HR2-902-12/StockOptions HEPES #12 pH 7.9, 185 ml
HR2-902-12/StockOptions HEPES #12 pH 7.9, 185 ml. Please note items from this supplier are non-returnable.
Bioworld MOPS-SDS Running Buffer (20X), 1 L
MOPS-SDS Running Buffer for Protein Gel electrophoresisSDS running buffer with MOPS and Tris (pH 7.7)20X concentrationAll bioWORLD running buffers are prepared with high quality reagentsSpecifications:Ready to use, just dilute in distilled water. Save time and ensure reproducible results.1X contains: 50mM MOPS, 50mM Tris base, 1mM EDTA, and 0.1% SDS
Sigma Aldrich Fine Chemicals Biosciences Hepes|7365-45-9 | MFCD00006158 | 100g
Hepes | Purity: 99.5% (titration) | MW: 238.3 | 7365-45-9 | MFCD00006158 | 100g
Medchemexpress LLC MES potassium | 39946-25-3 | 99.88% | 100 G
MES (2-Morpholinoethanesulphonic acid) potassium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5 to 7.0. It is a Good's buffer and is widely used in biochemistry and molecular biology experiments such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments (e.g., cell culture, enzyme activity determination, electrophoresis, protein studies)
- Zwitterionic buffer, provides effective buffering capacity around pH 6.15
- Pka value changes with temperature, requiring consideration for different temperatures
- Exhibits good buffering properties in biological experiments
- Shows poor affinity with metal ions (e.g., Mg2+, Ca2+, Mn2+), suitable for studies involving metal ions
Sigma Aldrich Fine Chemicals Biosciences MOPS BioUltra, for molecular biology, >=99.5% (T) | 1132-61-2 | MFCD00006183 | 100G
MOPS BioUltra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 100G
![Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659862.jpg-250.jpg)
Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 100 G
PIPES Disodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) disodium salt) is a biochemical reagent widely used in laboratory buffer solutions. This 100g formulation is perfect for maintaining a stable pH in various biological and biochemical assays. The disodium salt form provides a slightly stronger buffering capacity compared to its other forms, making it ideal for sensitive experiments such as protein electrophoresis and enzyme activity assays. It helps maintain a consistent pH of approximately 7.0, which is essential for stabilizing biological molecules during lab procedures. PIPES disodium salt is a reliable buffer in cell culture, microbiology, and biochemical studies.
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-5000.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 5 Kilograms
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.